Open Positions

29-Oct-2021

Genome Editing Manager

Faculty of Arts and Sciences

 

Job Summary

We seek an experienced and rigorous stem cell biologist and genetic engineer to lead efforts and oversee operations of genome editing of pluripotent stem cells to further the research goals of multiple projects in the Melton Lab. 

Job-Specific Responsibilities

This individual will implement the best CRISPR design and targeting strategy for each project (knock-out, knock-in or gene reporter) and will test new or alternative techniques to improve the generation of genetically modified ES/iPS lines. He/she will be actively involved in the design and generation of gene targeting vectors using recombinant DNA technology. The successful candidate will perform quality control of the engineered cell lines and perform directed differentiation towards the beta cell lineage. He/she will closely work with the foundry team to improve cell production of the genetically modified lines, generate standard operating procedures for differentiation and expand and distribute the engineered pluripotent stem cell line. He/she will train new personnel, assign projects and oversee the day to day activities of the assigned research staff. In addition, he/she will work closely with a group of talented graduate students and postdoctoral fellows to help design and construct cell lines. Hence, this role requires, in addition to technical expertise in stem cell culture and gene editing, effective communication with multiple personnel and excellent critical thinking and organizational skills.

Basic Qualifications

PhD degree in molecular biology or closely related field. A minimum of seven years of extensive directly related research are required. 

Additional Qualifications and Skills

The successful applicant will be a well-organized team player with stem cell tissue culture and CRISPR modification of pluripotent cell lines experience. He/sh must have a PhD in cell biology or related field with a focus on developmental and stem cell biology, and have a record of postdoctoral research accomplishments and publications.  

Additional Information

This is a two year grant funded term position with the possibility of extension based on satisfactory performance and continued availability of funding.


The FAS and HSCRB are committed to cultivating not only the diversity of our faculty, staff, and students but also in developing an inclusive culture that is vibrant, engaging and encouraging of innovation as well as intellectual debate. We believe creating and maintaining an inclusive workplace allows employees from all backgrounds and walks of life to achieve their fullest potential. We also believe an inclusive culture is one that accepts, values and views as strength the difference we all bring to the workplace. 

COVID Vaccination Policy

Harvard will require COVID vaccination for all Harvard community members who will have any on-campus presence. Individuals may claim exemption from the vaccine requirement for medical or religious reasons. More information regarding the University’s COVID vaccination requirement and exceptions may be found at the University’s “COVID-19 Vaccine Information” webpage: http://www.harvard.edu/coronavirus/covid-19-vaccine-information/.


Harvard offers an outstanding benefits package including:

  • Time Off: 3- 4 weeks paid vacation, paid holiday break, 12 paid sick days, 12.5 paid holidays, and 3 paid personal days per year.

  • Medical/Dental/Vision: We offer a variety of excellent medical plans, dental & vision plans, all coverage begins as of your start date.

  • Retirement: University-funded retirement plan with full vesting after 3 years of service.

  • Tuition Assistance Program: Competitive tuition assistance program, $40 per class at the Harvard Extension School and discounted options through participating Harvard grad schools. 

  • Transportation: Harvard offers a 50% discounted MBTA pass as well as additional options to assist employees in their daily commute.

  • Wellness Options: Harvard offers programs and classes at little or no cost, including stress management, massages, nutrition, meditation and complimentary health services.

  • Harvard access to athletic facilities, libraries, campus events and many discounts throughout metro Boston.Harvard offers an outstanding benefits package including:

  • Time Off: 3- 4 weeks paid vacation, paid holiday break, 12 paid sick days, 12.5 paid holidays, and 3 paid personal days per year.

  • Medical/Dental/Vision: We offer a variety of excellent medical plans, dental & vision plans, all coverage begins as of your start date.

  • Retirement: University-funded retirement plan with full vesting after 3 years of service.

  • Tuition Assistance Program: Competitive tuition assistance program, $40 per class at the Harvard Extension School and discounted options through participating Harvard grad schools. 

  • Transportation: Harvard offers a 50% discounted MBTA pass as well as additional options to assist employees in their daily commute.

  • Wellness Options: Harvard offers programs and classes at little or no cost, including stress management, massages, nutrition, meditation and complimentary health services.

  • Harvard access to athletic facilities, libraries, campus events and many discounts throughout metro Boston/

 

For more information: https://bit.ly/3CoTTwL

 

 

 

18-Oct-2021

Research Assistant II Lab

Faculty of Arts and Sciences

 

Job Summary

The RAII will be part of a team of 2-4 research staff of the Foundry team within Melton Lab, responsible for the specialized production of human islet cells from stem cells. 

Job-Specific Responsibilities

The RAII will be responsible with daily cultures of human pluripotent stem cells including maintenance, expansion and differentiation to various lineages. Under the direction of senior research staff in the Melton Lab, the RA will perform quantitative and qualitative assessments of beta cell directed differentiation using assays that include immunohistochemistry, FACS, gene expression, and physiological tests for beta cell function such as glucose tolerance testing (GTT), glucose stimulated insulin secretion (GSIS), ELISAs. Research assistants are expected to troubleshoot and co-develop standard protocols and quality control measures, and independently collect and record experimental data and update written protocols and methods based on findings.  Ability to work in a collaborative, team-oriented environment, and strong communication skills are required.

Basic Qualifications

College level coursework in biology or closely related field is required as is two years tissue of work experience with cell and tissue culture and sterile laboratory techniques in a biological research laboratory. 

Additional Qualifications and Skills

Bachelor’s degree in biology or closely related field. Experience with culturing human pluripotent stem cells including maintenance, expansion and differentiation to various lineages is preferred.

Physical Requirements

This work requires to frequently stand, and to lift, carry, move and transport related laboratory supplies.

 

Working Conditions

Harvard University COVID Policy
Harvard will require COVID vaccination for all Harvard community members who will have any on-campus presence. Individuals may claim exemption from the vaccine requirement for medical or religious reasons. More information regarding the University’s COVID vaccination requirement and exceptions may be found at the University’s “COVID-19 Vaccine Information” webpage: http://www.harvard.edu/coronavirus/covid-19-vaccine-information/.

Additional Information

This position requires a strong commitment and the willingness to work irregular hours, some holiday and weekend coverage will be required. This is a grant-funded position with a 2 year commitment preferred and the possibility of extension based on satisfactory performance and continued availability of funding. 

 

For more information: https://bit.ly/3iZOZxW