MELTON LAB
Selected Publications
Leite N, Pelayo G, Melton DA.
Stem Cell Report. 2022 Apr;12;17(4):766-774. doi:10.1016/j.stemcr.2022.01.018. Epub 2022 Mar 3.
Cell maturation: Hallmarks, triggers, and manipulation.
Alvarez-Dominguez J, Melton DA.
Cell. 2022 Jan;185(2):235-249. doi:10.1016/j.cell.2021.12.012.
Retrieval and purification of human β cells from stem cell-derived islet engrafted mice.
Kenty JHR, Pelayo G, Leite N.
STAR Protoc. 2021 Jul;15;2(3):100675. doi:10.1016/j.xpro.2021.100675.
Genetic Approaches to Attain Hypo-immunogenic Human Stem Cell Derived Islets for Transplantation
Sintov E, Rosado-Olivieri E, Leite N, Straubhaar J, Veres A, Boulanger K, Brehm M, Melton DA.
Transplantation. 2021 Dec;105(12 suppl 1): S13. doi:10.1097/01.tp.0000804444.63104.17.
Alagpulinsa D, Jei L, Pop R, Veres A, Deng H, Zhang J, Kawai K, Collin S, Sobell D, Chapin M, Verill D, Melton DA, Markmann J, Poznansky M.
Transplantation. 2021 Dec;105(12 suppl 2): S16. doi:10.1097/01.tp.0000804360.12644.62.
Engineering Immune-evasive Human Stem Cell-derived Islet Cells
Gerace D, Kenty JHR, Melton DA.
Transplantation. 2021 Dec;105(12 suppl 1): S13. doi:10.1097/01.tp.0000804344.89342.14.
A human ESC line for efficient CRISPR editing of pluripotent stem cells
Sintov E, Gerace D, Melton DA.
Stem Cell Res. 2021 Nov;57:102591. doi.org/10.1016/j.scr.2021.102591.
Gerace D, Boulanger KR, Kenty JHR, Melton DA.
Stem Cell Res. 2021 May;53:102371. doi: 10.1016/j.scr.2021.102371. Epub 2021 Apr 27.
Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells.
Leite NC, Sintov E, Meissner TB, Brehm MA, Greiner DL, Harlan DM, Melton DA.
Cell Rep. 2020 Jul 14;32(2):107894. doi: 10.1016/j.celrep.2020.107894.
Purification of Live Stem-Cell-Derived Islet Lineage Intermediates
Niloofar Rasouli, Douglas A Melton, Juan R Alvarez-Dominguez
Curr Protoc Stem Cell Biol. 2020 Jun;53(1):e111. doi: 10.1002/cpsc.111.
Glucose Response by Stem Cell-Derived β Cells In Vitro Is Inhibited by a Bottleneck in Glycolysis
Jeffrey C Davis, Tiago C Alves, Aharon Helman, Jonathan C Chen, Jennifer H Kenty, Rebecca L Cardone, David R Liu, Richard G Kibbey, Douglas A Melton
Cell Rep. 2020 May 12;31(6):107623. doi: 10.1016/j.celrep.2020.107623.
A Method for the Generation of Human Stem Cell-Derived Alpha Cells
Quinn P Peterson, Adrian Veres, Lihua Chen, Michael Q Slama, Jennifer H R Kenty, Shaimaa Hassoun, Matthew R Brown, Haiqiang Dou, Caden D Duffy, Quan Zhou, Aleksey V Matveyenko, Björn Tyrberg, Maria Sörhede-Winzell, Patrik Rorsman, Douglas A Melton
Nat Commun. 2020 May 7;11(1):2241. doi: 10.1038/s41467-020-16049-3
A Nutrient-Sensing Transition at Birth Triggers Glucose-Responsive Insulin Secretion
Aharon Helman, Andrew L Cangelosi, Jeffrey C Davis, Quan Pham, Arielle Rothman, Aubrey L Faust, Juerg R Straubhaar, David M Sabatini, Douglas A Melton
Cell Metab. 2020 May 5;31(5):1004-1016.e5. doi: 10.1016/j.cmet.2020.04.004.
A Stem Cell Approach to Cure Type 1 Diabetes
Aharon Helman, Douglas A Melton
Cold Spring Harb Perspect Biol. 2020 Mar 2:a035741. doi: 10.1101/cshperspect.a035741
Stephanie L Tsai, Clara Baselga-Garriga, Douglas A Melton
Elife. 2020 Jan 14;9:e50765. doi: 10.7554/eLife.50765.
Identification of a LIF-Responsive, Replication-Competent Subpopulation of Human β Cells
Edwin A Rosado-Olivieri, Idil I Aigha, Jennifer H Kenty, Douglas A Melton
Cell Metab. 2020 Feb 4;31(2):327-338.e6. doi: 10.1016/j.cmet.2019.12.009. Epub 2020 Jan 9.
Circadian Entrainment Triggers Maturation of Human In Vitro Islets
Juan R Alvarez-Dominguez, Julie Donaghey, Niloofar Rasouli, Jennifer H R Kenty, Aharon Helman, Jocelyn Charlton, Juerg R Straubhaar, Alexander Meissner, Douglas A Melton
Cell Stem Cell. 2020 Jan 2;26(1):108-122.e10. doi: 10.1016/j.stem.2019.11.011. Epub 2019 Dec 12.
Jeffrey C Davis, Aharon Helman, José Rivera-Feliciano, Christine M Langston, Elise N Engquist, Douglas A Melton
Curr Protoc Stem Cell Biol. 2019 Dec;51(1):e99. doi: 10.1002/cpsc.99.
Rosado-Olivieri EA, Anderson K , Kenty JH, Melton DA
Nat Commun. 2019 Apr 1;10(1):1464. doi: 10.1038/s41467-019-09404-6.
Wnt Signaling Separates the Progenitor and Endocrine Compartments During Pancreas Development
Nadav Sharon, Jordan Vanderhooft, Juerg Straubhaar, Jonas Mueller, Raghav Chawla, Quan Zhou, Elise N Engquist, Cole Trapnell, David K Gifford, Douglas A Melton
Cell Rep. 2019 May 21;27(8):2281-2291.e5. doi: 10.1016/j.celrep.2019.04.083.
Charting Cellular Identity During Human in Vitro β-cell Differentiation
Adrian Veres, Aubrey L Faust, Henry L Bushnell, Elise N Engquist, Jennifer Hyoje-Ryu Kenty, George Harb, Yeh-Chuin Poh, Elad Sintov, Mads Gürtler, Felicia W Pagliuca, Quinn P Peterson, Douglas A Melton
Peterson QP, Melton DA.
Nature. 2019 May;569(7756):368-373. doi: 10.1038/s41586-019-1168-5. Epub 2019 May 8.
Generation of Hypoimmunogenic Human Pluripotent Stem Cells
Xiao Han, Mengning Wang, Songwei Duan, Paul J Franco, Jennifer Hyoje-Ryu Kenty, Preston Hedrick, Yulei Xia, Alana Allen, Leonardo M R Ferreira, Jack L Strominger, Douglas A Melton, Torsten B Meissner , Chad A Cowan
Proc Natl Acad Sci U S A. 2019 May 21;116(21):10441-10446. doi: 10.1073/pnas.1902566116. Epub 2019
Alagpulinsa DA , Cao JJL, Driscoll RK, Sîrbulescu RF, Penson MFE, Sremac M, Engquist EN , Brauns TA, Markmann JF, Melton DA, Poznansky MC
Am J Transplant. 2019 Jul;19(7):1930-1940. doi: 10.1111/ajt.15308. Epub 2019 Mar 25.
Blastemal Progenitors Modulate Immune Signaling During Early Limb Regeneration
Tsai SL, Baselga-Garriga C, Melton DA
Development. 2019 Jan 2;146(1):dev169128. doi: 10.1242/dev.169128.
Zhou Q, Melton DA
Nature. 2018 Aug;560(7720):E34. doi: 10.1038/s41586-018-0294-9.
Baron M, Veres A, Wolock SL, Faust AL, Gaujoux R, Vetere A, Ryu JH, Wagner BK, Shen-Orr SS, Klein AM, Melton DA, Yanai I.
Cell Syst. 2016 Oct 26;3(4):346-360.e4. doi: 10.1016/j.cels.2016.08.011. Epub 2016 Sep 22.
Generation of stem cell-derived β-cells from patients with type 1 diabetes.
Millman JR, Xie C, Van Dervort A, Gürtler M, Pagliuca FW, Melton DA.
Nat Commun. 2016 May 10;7:11463. doi: 10.1038/ncomms11463.
Vegas AJ, Veiseh O, Gürtler M, Millman JR, Pagliuca FW, Bader AR, Doloff JC, Li J, Chen M, Olejnik K, Tam HH, Jhunjhunwala S, Langan E, Aresta-Dasilva S, Gandham S, McGarrigle JJ, Bochenek MA, Hollister-Lock J, Oberholzer J, Greiner DL, Weir GC, Melton DA, Langer R, Anderson DG.
Nat Med. 2016 Mar;22(3):306-11. doi: 10.1038/nm.4030. Epub 2016 Jan 25.
Applied Developmental Biology: Making Human Pancreatic Beta Cells for Diabetics.
Melton DA.
Curr Top Dev Biol. 2016;117:65-73. doi: 10.1016/bs.ctdb.2015.11.013. Epub 2016 Jan 20. Review.
PMID: 26969972
Kenty JH, Melton DA.
PLoS One. 2015 Apr 8;10(4):e0122044. doi: 10.1371/journal.pone.0122044. eCollection 2015.
PMID: 25853429
Modeling human nutrition using human embryonic stem cells.
Ben-Zvi D, Melton DA.
Cell. 2015 Mar 26;161(1):12-17. doi: 10.1016/j.cell.2015.02.039.
PMID:25815980
Generation of functional human pancreatic β cells in vitro.
Pagliuca FW, Millman JR, Gürtler M, Segel M, Van Dervort A, Ryu JH, Peterson QP, Greiner D, Melton DA.
Cell. 2014 Oct 9;159(2):428-39. doi: 10.1016/j.cell.2014.09.040.
PMID: 25303535
Reversal of β cell de-differentiation by a small molecule inhibitor of the TGFβ pathway.
Blum B, Roose AN, Barrandon O, Maehr R, Arvanites AC, Davidow LS, Davis JC, Peterson QP, Rubin LL, Melton DA.
Elife. 2014 Sep 16;3:e02809. doi: 10.7554/eLife.02809.
PMID: 25233132
MARIS: method for analyzing RNA following intracellular sorting.
Hrvatin S, Deng F, O'Donnell CW, Gifford DK, Melton DA.
PLoS One. 2014 Mar 3;9(3):e89459. doi: 10.1371/journal.pone.0089459. eCollection 2014.
PMID:24594682
In vivo reprogramming of pancreatic acinar cells to three islet endocrine subtypes.
Li W, Nakanishi M, Zumsteg A, Shear M, Wright C, Melton DA, Zhou Q.
Elife. 2014 Jan 1;3:e01846. doi: 10.7554/eLife.01846.
PMID: 24714494