Selected Publications

Genetic manipulation of stress pathways can protect stem-cell-derived islets from apoptosis in vitro.

Leite N, Pelayo G, Melton DA.

Stem Cell Report. 2022 Apr;12;17(4):766-774. doi:10.1016/j.stemcr.2022.01.018. Epub 2022 Mar 3.

Cell maturation: Hallmarks, triggers, and manipulation.

Alvarez-Dominguez J, Melton DA.

Cell. 2022 Jan;185(2):235-249. doi:10.1016/j.cell.2021.12.012.

Retrieval and purification of human β cells from stem cell-derived islet engrafted mice.

Kenty JHR, Pelayo G, Leite N.

STAR Protoc. 2021 Jul;15;2(3):100675. doi:10.1016/j.xpro.2021.100675.

Genetic Approaches to Attain Hypo-immunogenic Human Stem Cell Derived Islets for Transplantation

Sintov E, Rosado-Olivieri E, Leite N, Straubhaar J, Veres A, Boulanger K, Brehm M, Melton DA.

Transplantation. 2021 Dec;105(12 suppl 1): S13. doi:10.1097/01.tp.0000804444.63104.17.

Long-term Functional Survival of Human Stem Cell-derived Islets Microencapsulated in Alginate With CXCL12 in Non-human Primates Without Immunosuppression.

Alagpulinsa D, Jei L, Pop R, Veres A, Deng H, Zhang J, Kawai K, Collin S, Sobell D, Chapin M, Verill D, Melton DA, Markmann J, Poznansky M.

Transplantation. 2021 Dec;105(12 suppl 2): S16. doi:10.1097/01.tp.0000804360.12644.62.

Engineering Immune-evasive Human Stem Cell-derived Islet Cells

Gerace D, Kenty JHR, Melton DA.

Transplantation. 2021 Dec;105(12 suppl 1): S13. doi:10.1097/01.tp.0000804344.89342.14.

A human ESC line for efficient CRISPR editing of pluripotent stem cells

Sintov E, Gerace D, Melton DA.

Stem Cell Res. 2021 Nov;57:102591. doi.org/10.1016/j.scr.2021.102591.

A Stem Cell Approach to Cure Type 1 Diabetes

Aharon Helman, Douglas A Melton

Cold Spring Harb Perspect Biol. 2020 Mar 2:a035741. doi: 10.1101/cshperspect.a035741

Blastemal Progenitors Modulate Immune Signaling During Early Limb Regeneration

Tsai SL, Baselga-Garriga C, Melton DA

Development. 2019 Jan 2;146(1):dev169128. doi: 10.1242/dev.169128.

Pancreas Regeneration

Zhou Q, Melton DA

Nature. 2018 Aug;560(7720):E34. doi: 10.1038/s41586-018-0294-9.

A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure.

Baron M, Veres A, Wolock SL, Faust AL, Gaujoux R, Vetere A, Ryu JH, Wagner BK, Shen-Orr SS, Klein AM, Melton DA, Yanai I.

Cell Syst. 2016 Oct 26;3(4):346-360.e4. doi: 10.1016/j.cels.2016.08.011. Epub 2016 Sep 22.

Generation of stem cell-derived β-cells from patients with type 1 diabetes.

Millman JR, Xie C, Van Dervort A, Gürtler M, Pagliuca FW, Melton DA.

Nat Commun. 2016 May 10;7:11463. doi: 10.1038/ncomms11463.

Long-term glycemic control using polymer-encapsulated human stem cell-derived beta cells in immune-competent mice.

Vegas AJ, Veiseh O, Gürtler M, Millman JR, Pagliuca FW, Bader AR, Doloff JC, Li J, Chen M, Olejnik K, Tam HH, Jhunjhunwala S, Langan E, Aresta-Dasilva S, Gandham S, McGarrigle JJ, Bochenek MA, Hollister-Lock J, Oberholzer J, Greiner DL, Weir GC, Melton DA, Langer R, Anderson DG.

Nat Med. 2016 Mar;22(3):306-11. doi: 10.1038/nm.4030. Epub 2016 Jan 25. 

Applied Developmental Biology: Making Human Pancreatic Beta Cells for Diabetics.

Melton DA.

Curr Top Dev Biol. 2016;117:65-73. doi: 10.1016/bs.ctdb.2015.11.013. Epub 2016 Jan 20. Review.

PMID: 26969972

Testing pancreatic islet function at the single cell level by calcium influx with associated marker expression.

Kenty JH, Melton DA.

PLoS One. 2015 Apr 8;10(4):e0122044. doi: 10.1371/journal.pone.0122044. eCollection 2015.

PMID: 25853429

Modeling human nutrition using human embryonic stem cells.

Ben-Zvi D, Melton DA.

Cell. 2015 Mar 26;161(1):12-17. doi: 10.1016/j.cell.2015.02.039.

PMID:25815980

Generation of functional human pancreatic β cells in vitro.

Pagliuca FW, Millman JR, Gürtler M, Segel M, Van Dervort A, Ryu JH, Peterson QP, Greiner D, Melton DA.

Cell. 2014 Oct 9;159(2):428-39. doi: 10.1016/j.cell.2014.09.040.

PMID: 25303535

Reversal of β cell de-differentiation by a small molecule inhibitor of the TGFβ pathway.

Blum B, Roose AN, Barrandon O, Maehr R, Arvanites AC, Davidow LS, Davis JC, Peterson QP, Rubin LL, Melton DA.

Elife. 2014 Sep 16;3:e02809. doi: 10.7554/eLife.02809.

PMID: 25233132

MARIS: method for analyzing RNA following intracellular sorting.

Hrvatin S, Deng F, O'Donnell CW, Gifford DK, Melton DA.

PLoS One. 2014 Mar 3;9(3):e89459. doi: 10.1371/journal.pone.0089459. eCollection 2014.

PMID:24594682

In vivo reprogramming of pancreatic acinar cells to three islet endocrine subtypes.

Li W, Nakanishi M, Zumsteg A, Shear M, Wright C, Melton DA, Zhou Q.

Elife. 2014 Jan 1;3:e01846. doi: 10.7554/eLife.01846.

PMID: 24714494